| 1e ronde | ||||
| 06/09/1980 | Caesar (III) | SC Amersfoort (II) ** | 2-5 | |
| 06/09/1980 | De Graafschap (II) | Telstar (II) | 2-3 | |
| 06/09/1980 | DETO (III) | DS'79 (II) | 2-5 | |
| 06/09/1980 | FC Amsterdam (II) | Arnemuiden (IV) | 8-1 | |
| 06/09/1980 | FC Den Bosch'67 (II) | Rheden (III) | 3-0 | |
| 06/09/1980 | FC Vlaardingen'74 (II) | Xerxes (III) | 4-2 | |
| 06/09/1980 | FC Volendam (II) | Noordwijk (III) | 3-1 | |
| 06/09/1980 | Fortuna Sittard (II) ** | SC Cambuur (II) | 2-0 | |
| 06/09/1980 | Haarlem (II) | Vitesse (II) | 3-0 | |
| 06/09/1980 | ROHDA Raalte (III) *** | Eindhoven (II) | 1-1 | |
| 06/09/1980 | sc Heerenveen (II) | ACV (III) | 3-2 | |
| 06/09/1980 | SC Veendam (II) | Helmond Sport (II) | 2-0 | |
| 06/09/1980 | SVV (II) | DESK (III) | 5-1 | |
| 06/09/1980 | FC VVV (II) ** | SC Heracles'74 (II) | 3-2 | |
| 2e ronde | ||||
| 15/11/1980 | Ajax | SVV (II) | 3-0 | |
| 15/11/1980 | AZ'67 | sc Heerenveen (II) | 4-1 | |
| 15/11/1980 | DS'79 (II) | PSV | 0-1 | |
| 15/11/1980 | Excelsior | FC Vlaardingen'74 (II) ** | 2-2 | |
| 15/11/1980 | FC Den Bosch'67 (II) | FC Utrecht | 2-4 | |
| 15/11/1980 | FC Twente'65 | FC Volendam (II) | 1-0 | |
| 15/11/1980 | FC Wageningen | FC Den Haag | 4-1 | |
| 15/11/1980 | Feyenoord | SC Veendam (II) | 5-1 | |
| 15/11/1980 | Fortuna Sittard (II) | Roda JC | 1-2 | |
| 15/11/1980 | Go Ahead Eagles | ROHDA Raalte (III) | 9-3 | |
| 15/11/1980 | Haarlem (II) *** | NAC | 2-2 | |
| 15/11/1980 | MVV | FC Amsterdam (II) | 3-0 | |
| 15/11/1980 | NEC | FC Groningen | 0-1 | |
| 15/11/1980 | SC Amersfoort (II) | FC VVV (II) *** | 2-2 | |
| 15/11/1980 | Sparta Rotterdam | PEC Zwolle *** | 2-2 | |
| 15/11/1980 | Telstar (II) | Willem II (II) *** | 2-2 | |
| 3e ronde | ||||
| 24/01/1981 | Ajax | FC Twente'65 | 5-1 | |
| 24/01/1981 | AZ'67 | FC Vlaardingen'74 (II) | 5-2 | |
| 24/01/1981 | FC Groningen | FC VVV (II) | 3-2 | |
| 04/02/1981 | FC Wageningen | PEC Zwolle | 0-3 | |
| 24/01/1981 | Feyenoord | Haarlem (II) *** | 3-3 | |
| 24/01/1981 | Go Ahead Eagles | MVV | 3-1 | |
| 24/01/1981 | PSV | Roda JC | 4-0 | |
| 24/01/1981 | Willem II (II) | FC Utrecht | 2-1 | |
| Kwartfinale | ||||
| 25/02/1981 | Ajax | FC Groningen | 3-0 | |
| 25/02/1981 | Go Ahead Eagles | PEC Zwolle | 1-1 | |
| 25/02/1981 | Haarlem (II) | AZ'67 | 1-1 | |
| 25/02/1981 | Willem II (II) | PSV | 0-1 | |
| 01/04/1981 | AZ'67 | Haarlem (II) | 3-1 | |
| 01/04/1981 | FC Groningen | Ajax | 1-1 | |
| 01/04/1981 | PEC Zwolle | Go Ahead Eagles | 1-2 | |
| 01/04/1981 | PSV | Willem II (II) | 2-0 | |
| Halve Finale | ||||
| 14/04/1981 | Go Ahead Eagles | AZ'67 | 2-2 | |
| 14/04/1981 | PSV | Ajax | 2-2 | |
| 12/05/1981 | Ajax | PSV | 2-1 | |
| 12/05/1981 | AZ'67 | Go Ahead Eagles | 6-1 | |
| Finale | ||||
| 28/05/1981 | Ajax | AZ'67 | 1-3 | 0-1 Pier Tol, 1-1 Gerald Vanenburg, 1-2 Ronald Spelbos, 1-3 Kristen Nygaard Gespeeld in Amsterdam (Olympisch Stadion) |
